Coding

Part:BBa_K220003:Design

Designed by: Sarah Sandock   Group: iGEM09_Wisconsin-Madison   (2009-10-18)


ADP - sugar Pyrophosphatase (NudF)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 286
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Length: 53 Fwd: 5'- CGA TCT AGA AGG AGG ATA TAT TAA TGA AAT CAT TAG AAG AAA AAA CAA TTG CC -3'

Length: 38 Rev: 5'- TCA CTG CAG ATC ACT AGT TCA TTT TTG TGC TTG GAG CG -3'



Source

Gene isolation E.Coli Strain K12

References